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UV/VIS absorbance measurements



Absorbance measurements in fluids can be done in different setups and wavelength ranges, like with fiber optic dip probes or flow cells for in-line absorbance or a cuvette holder for absorption measurement in a sample. For UV/VIS measurements a spectrometer can be configured with a range from 200 to 1100 nm and a spectral resolution of 1.4 nm FWHM. Further a combined deuterium-halogen light source is needed. For the different applications different probes can be used. A typical setup for absorption measurement is shown below.

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Components used in the absorption measurement setup are depicted in the following table:

 

In-line Absorption with flow cell

In-line Absorption with Dip probe

Absorption with cuvette holder

Spectrometer

 

AvaSpec-2048-USB2, grating UA (200-1100nm), DUV, slit-25, DCL-UV , OSC-UA
AvaSpec-2048x14, grating UA (200-1100nm), slit-25, OSC-UA 

Software

AvaSoft-Full optional AvaSoft-CHEM

Light source

AvaLight-DH-S-BAL Balanced Deuterium-Halogen light source

Fiber optics

2 pcs. FC-UV200-2-SR fiber cable 200µm UV/VIS, solarization resistant, 2m, SMA

FDP-7UV200-2-VAR Transmission dip probe with variable path length, with 6x200µm illumination fibers, 1 read fiber, UV/VIS, 2m, SMA

2 pcs. FC-UV200-2-SR fiber cable 200µm UV/VIS, solarization resistant, 2m, SMA

Accessories

1/4"or 1/2"flow cell

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CUV-UV/VIS cuvette holder